anti β gal (Proteintech)
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Anti β Gal, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2+gal/Beta+galactosidase+Antibody/pmc13617884-247-56-57
Average 95 stars, based on 86 article reviews
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Next-Generation Sequencing:Article Title: Targeting the cGAS-STING pathway alleviates neuroinflammation and cognitive impairment induced by chronic infection of Toxoplasma gondii Article Snippet: Brain tissues were dehydrated in 30% sucrose solutions, embedded in tissue embedding medium (epredia, Neg-50) and sectioned into 20 μm coronal sections. .. Sections were then blocked with a buffer prepared by mixing 10% BSA, normal goat serum (NGS), PBS and 0.6% Triton X-100 at a volume ratio of 1:1:3:5 ; thereafter, they were incubated overnight at 4 °C with the following primary antibodies: anti-GFAP (Servicebio, GB12096-100, 1:500 dilution), anti-IBA1 (Abcam, ab178847, 1:300 dilution), anti-NeuN (Proteintech, 26975-1-AP, 1:500 dilution), Incubation:Article Title: Targeting the cGAS-STING pathway alleviates neuroinflammation and cognitive impairment induced by chronic infection of Toxoplasma gondii Article Snippet: Brain tissues were dehydrated in 30% sucrose solutions, embedded in tissue embedding medium (epredia, Neg-50) and sectioned into 20 μm coronal sections. .. Sections were then blocked with a buffer prepared by mixing 10% BSA, normal goat serum (NGS), PBS and 0.6% Triton X-100 at a volume ratio of 1:1:3:5 ; thereafter, they were incubated overnight at 4 °C with the following primary antibodies: anti-GFAP (Servicebio, GB12096-100, 1:500 dilution), anti-IBA1 (Abcam, ab178847, 1:300 dilution), anti-NeuN (Proteintech, 26975-1-AP, 1:500 dilution), |
![( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using <t>IgG</t> (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_35/10__64898_slash_2026__04__02__716135/10__64898_slash_2026__04__02__716135___F4.large.jpg)